Advanced Flow Cytometry Laboratory (Officer In-charge: Prof.Ravi V)
The flow cytometry lab is equipped with an advanced four laser BD FACS Aria III cell sorter and a three laser FACS Verse analyzer, which enable sorting and analysis of single cells based on multiple parameters respectively. Students / researchers from departments of Neurovirology (4), Neuropathology (1) etc use this machine for their research work under the supervision of a Senior Scientific Officer.
Completed research activities in this lab includes the study of innate immune mechanisms in Japanese Encephalitis Virus Infection and ongoing research activities include: a)A Comparative Study of Innate and Cell Mediated Immune Responses in Human Immunodeficiency virus infected individuals with and without Neurotuberculosis (b) Clinical and immunological profile of patients with Subacute Sclerosing Pan Encephalitis (c) Flow cytometry based detection of dysferlin protein expression in CD14 positive monocytes from peripheral blood samples of patients with Dysferlinopathy(d) Analysis of brain infiltrating lymphocytes in mouse brain following vaccination against Rabies or infection with Rabies virus.(e) Analysis of cellular immune responses in patients with Schizophrenia.
The laboratory has 4 ongoing funded projects. More than 10 researchers including PhD scholars( including external PhD student from Liverpool university), Post doctoral fellows and faculty from NIMHANS are currently using the lab facility. The first ‘Hands-on Workshop on Flow Cytometry Applications’ was conducted by the laboratory between 16th to 18th June 2014. About 15 participants attended the workshop.
